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Item #:
86988418
Phosphate Buffer, pH 7.0 [0.5 M] - 500 ml
94% of respondents would recommend this to a friend
Item #:
86988418
ISK 15369
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Phosphate buffer is used in immunochemical staining procedures and serves as an important fixative for light and electron microscopy.
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What Stands Out
Optimal pH Stability
Maintains a stable pH of 7.0, ensuring reliable results in biochemical and molecular applications, addressing common fluctuations in pH for consistent experimentation.
High Concentration
At 0.5 M concentration, this phosphate buffer provides sufficient buffering capacity, effectively preventing pH changes during reactions, which is crucial for sensitive applications.
Convenient Packaging
Comes in a 500 ml bottle, offering optimal size for laboratory use, reducing the risk of waste and allowing easy storage for multiple applications.
Product Details
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.77 |
| Item Weight | 8 ounces (226.8 grams) |
| Brand | eCon Lab Supply |
Who Should Buy?
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Biology Laboratories
Ideal for experiments requiring stable pH, especially in cell culture and biochemical assays for consistent results.
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Educational Institutions
Perfect for teaching basic principles of buffering solutions in chemistry and biology courses for accurate experiments.
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Pharmaceutical Research
Essential for drug formulation studies that require precise pH control during stability and efficacy testing of compounds.
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Field Studies
Not suitable for outdoor or uncontrolled environments where precise pH monitoring and adjustments are challenging.
Product Description
Phosphate Buffer, pH 7.0 [0.5 M] - 500 ml
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Customer Questions & Answers
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Question:
What is a phosphate buffer, and how is it used?
Answer: A phosphate buffer is a solution that maintains a stable pH level, particularly at pH 7.0 in this case. It's commonly used in biological and chemical applications to provide an optimal environment for reactions. This is crucial in experiments involving enzymes and nucleic acids, where precise pH levels significantly influence performance and stability. -
Question:
What are the applications of a phosphate buffer in laboratories?
Answer: Phosphate buffers are widely used in various laboratory settings. They maintain a consistent pH in biological assays, tissue culture, and protein purification processes. Such stability is vital since many molecules, including proteins and enzymes, can denature or lose functionality outside their optimal pH range. Using this buffer helps ensure accurate and reproducible experimental results. -
Question:
Is the solution sterilized and ready to use directly from the bottle?
Answer: Yes, most commercially available phosphate buffers, like this one, are sterilized and prepared for direct use. This convenience means you can confidently apply it in sensitive experiments without needing to dilute or sterilize the buffer further, which saves time and reduces the risk of contamination. -
Question:
What is the significance of the pH level being 7.0?
Answer: A pH level of 7.0 indicates that the buffer is neutral, making it suitable for a wide range of biological applications. Many cellular and biochemical processes occur optimally at this pH level, ensuring that proteins and enzymes retain their functionality. It's a standard choice in molecular biology and biochemistry experiments where maintaining physiological conditions is critical. -
Question:
Can I use phosphate buffers for cell biology experiments?
Answer: Absolutely! Phosphate buffers are integral in cell biology experiments, particularly for maintaining the pH of culture media. They help create a stable environment for cells, which is essential for growth and experimentation. This is particularly important in studies involving primary cells, cell lines, or any assay requiring proper pH maintenance. -
Question:
What should I do if I need a different buffer pH range?
Answer: If you require a different pH range, you can adjust the phosphate buffer using acids or bases to fine-tune it. For example, to lower the pH, phosphoric acid can be added. However, it's essential to use precise measuring tools to ensure you achieve the desired pH without compromising the buffer's properties, thus maintaining experimental integrity. -
Question:
How should I store the phosphate buffer once opened?
Answer: Once opened, phosphate buffers should be stored in a cool, dark place, preferably in a tightly sealed container to prevent contamination. Protecting the buffer from light and heat helps maintain its effectiveness over time. Following proper storage guidelines ensures that the buffer remains stable and ready for immediate use when needed. -
Question:
Are there any safety precautions I should take?
Answer: While phosphate buffers are generally safe to handle, it is wise to use basic laboratory safety practices. Always wear gloves and goggles while handling any chemical solutions to minimize risk. Additionally, ensure adequate ventilation in your working area to prevent inhalation of vapors or aerosols that might be produced, especially when making adjustments to the buffer. -
Question:
What are the components of the 0.5 M phosphate buffer?
Answer: The 0.5 M phosphate buffer typically consists of a mixture of dihydrogen phosphate (KH2PO4) and hydrogen phosphate (Na2HPO4). This combination allows the buffer to effectively maintain pH while providing necessary ionic strength for biological reactions. Each component plays a critical role in creating the balance needed for various applications, ensuring reliable results. -
Question:
Where can I buy Phosphate Buffer, pH 7.0 [0.5 M] - 500 ml in Iceland?
Answer: You can purchase Phosphate Buffer, pH 7.0 [0.5 M] - 500 ml from Ubuy in Iceland. Ubuy offers a wide range of laboratory supplies, making it convenient to find this buffer solution along with other essential materials for your experiments.
eCon Lab Supply Buffers Editorial Review
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ISK 15369
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Features & Benefits
- Comprehensive range of biological buffers for research and diagnostics
- Important for protein research, molecular biology, and characterization studies
- Manufactured in ISO 9001 and 13485 certified facility in the USA
- Buffers are produced following GMP and quality control tests in a certified clean room
- Phosphate Buffer pH 6.5 [0.5 M] is ideal for immunochemical staining procedures
- Commonly used in surgeries to preserve living tissues
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